RASSF1 monoclonal antibody, clone 3F3
产品名称： RASSF1 monoclonal antibody, clone 3F3
英文名称： RASSF1 monoclonal antibody, clone 3F3
产品编号： MAB1056
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant RASSF1.
- Immunogen:
- Recombinant protein corresponding to full length human RASSF1.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG1, kappa
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Hypermethylation of RASSF1A in human and rhesus placentas.
Chiu RW, Chim SS, Wong IH, Wong CS, Lee WS, To KF, Tong JH, Yuen RK, Shum AS, Chan JK, Chan LY, Yuen JW, Tong YK, Weier JF, Ferlatte C, Leung TN, Lau TK, Lo KW, Lo YM.Am J Pathol. 2007 Mar;170(3):941-50.
- 2.
- Chromatin inactivation precedes de novo DNA methylation during the progressive epigenetic silencing of the RASSF1A promoter.
Strunnikova M, Schagdarsurengin U, Kehlen A, Garbe JC, Stampfer MR, Dammann R.Mol Cell Biol. 2005 May;25(10):3923-33.
- 3.
- The tumor suppressor RASSF1A in human carcinogenesis: an update.
Dammann R, Schagdarsurengin U, Seidel C, Strunnikova M, Rastetter M, Baier K, Pfeifer GP.Histol Histopathol. 2005 Apr;20(2):645-63.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of HEK 293 (1) , A - 549 (2) and HeLa (3) cells extracts were resolved by SDS - PAGE , transferred to PVDF membrane and probed with RASSF1 monoclonal antibody , clone 3F3 (1 : 1000) (Cat # MAB1056) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system.
- Entrez GeneID:
- 11186
- Gene Name:
- RASSF1
- Gene Alias:
- 123F2,NORE2A,RASSF1A,RDA32,REH3P21
- Gene Description:
- Ras association (RalGDS/AF-6) domain family member 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
- Other Designations:
- OTTHUMP00000195503,Ras association (RalGDS/AF-6) domain family 1,Ras association domain family 1,Ras association domain family protein 1,WUGSC:H_LUCA12.5,cardiac-specific ras association domain family 1 protein,pancreas-specific ras association domain fam