FITC标记的细胞分裂相关蛋白CSTF64抗体
产品名称： FITC标记的细胞分裂相关蛋白CSTF64抗体
英文名称： Anti-CstF-64/FITC
产品编号： HZ-6995R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-CstF-64/FITC Conjugated antibody
FITC标记的细胞分裂相关蛋白CSTF64抗体
| 产品编号 | bs-6995R-FITC |
| 英文名称 | Anti-CstF-64/FITC |
| 中文名称 | FITC标记的细胞分裂相关蛋白CSTF64抗体 |
| 别 名 | CF1 64 kDa subunit, tau variant; Cleavage stimulation factor 64 kDa subunit, tau variant; CSTF 64 kDa subunit, tau variant; KIAA0689; TauCstF64; CSTFT_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Q9H0L4/CstF-64 (51-150aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation. Function: May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs Subcellular Location: Nuclear Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 RRM (RNA recognition motif) domain. Database links: Entrez Gene: 1478 Human Omim: 300907 Human SwissProt: P33240 Human Unigene: 132370 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
mRNA前体的多腺苷化是需要多个蛋白因子的两步反应。第一步，多聚腺苷酸化底物的内切核切割，需要CstF（裂解刺激因子），由三个不同亚基组成的异三聚体。CSTF 64含有一个RNA结合结构域，负责CSTF的RNA结合活性。CSTF 64在所有体细胞中均有表达，在减数分裂前期和减数分裂中表达，而不是减数分裂生殖细胞。然而，CSTF 64的一个大变种，称为T CSTF 64，在睾丸减数分裂和减数分裂细胞中大量表达，在大脑中的程度较小，并促进多腺苷酸化的生殖细胞模式。编码CSTF 64的基因（指定的CSTF2）映射到X染色体，而T CSTF 64由常染色体基因编码。在B细胞活化过程中CSTF 64浓度的增加从膜结合到分泌形式切换了IgM重链mRNA表达，提示CSTF 64在B细胞分化过程中调节IgM重链表达起着关键作用。