白血病抑制因子受体抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
白血病抑制因子受体抗体

白血病抑制因子受体抗体

商家询价

产品名称： 白血病抑制因子受体抗体

英文名称： LIFR

产品编号： 1414

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： WB ELISA IHC-P IHC-F Flow-Cyt IF

上海雅吉生物科技有限公司
  • 联系人 :
  • 地址 : 上海市闵行区元江路5500号第1幢5658室
  • 邮编 :
  • 所在区域 : 上海
  • 电话 : 158****3937 点击查看
  • 传真 : 点击查看
  • 邮箱 : yajikit@163.com
进入展台

 

中文名称 白血病抑制因子受体抗体
别    名 CD 118; leukemia inhibitory factor receptor; SWS; SJS2; STWS; CD118; LIF-R; FLJ98106; FLJ99923; LIFR; Leukemia inhibitory factor receptor alpha; LIF R; LIF receptor; LIFR_RAT.  

 

研究领域 细胞生物  免疫学  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,  (predicted: Mouse, Rat, Dog, Cow, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 117kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from the middle of rat LIFR:451-580/1093 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍
This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene.

Function:
Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells (By similarity).

Subunit:
"Heterodimer composed of LIFR and IL6ST. The heterodimer formed by LIFR and IL6ST interacts with the complex formed by CNTF and CNTFR.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 6 fibronectin type-III domains.

SWISS:
P42702

Gene ID:
81680

Database links:

Entrez Gene: 3977 Human

Entrez Gene: 16880 Mouse

Entrez Gene: 81680 Rat

Omim: 151443 Human

SwissProt: P42702 Human

SwissProt: P42703 Mouse

SwissProt: O70535 Rat

Unigene: 133421 Human

Unigene: 149720 Mouse

Unigene: 14529 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-LIFR (bs-1458R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 117 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LIFR/CD118 Polyclonal Antibody, Unconjugated(bs-1458R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LIFR/CD118 Polyclonal Antibody, Unconjugated(bs-1458R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U937(blue).
Primary Antibody:Rabbit Anti-LIFR antibody(bs-1458R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1458R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

​