SGK1 (S422D) (Human) Recombinant Protein
产品名称： SGK1 (S422D) (Human) Recombinant Protein
英文名称： SGK1 (S422D) (Human) Recombinant Protein
产品编号： P5750
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
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- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Human SGK1 (NP_005618.2, 61 a.a. - 431 a.a.) S422D mutant partial recombinant protein with GST tag co-expressed with His-tagged PDPK1 (NP_002604.1, 1 a.a. - 556 a.a.) full length recombinant protein expressed in Baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 69
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 73 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : SGKtide. ATP: 100 µM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- SDS-PAGE Stained with Coomassie Blue
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 6446
- Protein Accession#:
- NP_005618.2
- Gene Name:
- SGK1
- Gene Alias:
- SGK
- Gene Description:
- serum/glucocorticoid regulated kinase 1
- Omim ID:
- 602958
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000017247,serine/threonine protein kinase SGK