水通道蛋白-9抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
水通道蛋白-9抗体

水通道蛋白-9抗体

商家询价

产品名称: 水通道蛋白-9抗体

英文名称: AQP9

产品编号: 2260

产品价格: null

产品产地: 上海

品牌商标: 雅吉

更新时间: null

使用范围: WB ELISA Flow-Cyt

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中文名称 水通道蛋白-9抗体
别    名 aquaporin Protein-9; Aquaporin 9; AQP 9; AQP9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1; Aquaporin-9; AQP-9; Aquaglyceroporin-9; AQP9_HUMAN.  

 

抗体来源 Rabbit 

克隆类型 Polyclonal 

交叉反应 Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, ) 

产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 32kDa 

细胞定位 细胞膜  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human AQP9:201-295/295  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. A new member of the AQP family, AQP9, has been cloned from human leukocytes. It is homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and in lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it does not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to b

产品图片
Sample:Intestinal(Mouse) lysate at 30 ug
Primary: Anti-AQP9 (bs-2060R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 32 kD
Observed band size : 32 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-AQP9 (bs-2060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
Sample:Liver(Mouse) lysate at 30 ug
Primary: Anti-AQP9 (bs-2060R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 32 kD
Observed band size : 32 kD
Blank control: A549(blue).
Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (bs-2060R /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-2060R/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.