BspQI GMP-grade (10 U/μL)
产品名称： BspQI GMP-grade (10 U/μL)
英文名称： BspQI GMP-grade (10 U/μL)
产品编号： 10664ES76
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:18:22
使用范围： null
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Product Information
|
Product name |
Cat# |
Size |
Price (￥) |
|
BspQI GMP-grade (10 U/μL) |
10664ES76 |
500 U |
365 |
|
10664ES86 |
2,500 U |
1385 |
|
|
10664ES92 |
10,000 U |
4155 |
Product Description
This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5-GCTCTTCN1/N4-3. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.
This product is produced in accordance with GMP process requirements and provided in a liquid form.
Product Properties
|
Source |
Recombinant E. coli with BspQI gene |
|
Reaction Temperature |
50℃ |
|
Storage Conditions |
20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol |
|
Unit Definition |
1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system |
Contents
|
Contents No. |
Name |
Cat#./Specification |
||
|
10664ES76 (500 U) |
10664ES80 (2,500 U) |
10664ES92 (10,000 U) |
||
|
10664 |
BspQI GMP-grade (10 U/μL) |
50 μL |
250 μL |
1 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Applications
1. Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
2. Digestion of DNA to obtain specific sticky ends.
Experimental methods
50 μL reaction system
This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.
1）Add the following components in sequence:
|
Components |
Volume |
|
Plasmid DNA |
1-2 μg |
|
10× Digestion Buffer 3 |
5.0 μL |
|
BspQI (10 U/μL) |
1.0 μL |
|
RNase-free ddH2O |
Up to 50 μL |
Note: 10× Digestion Buffer 3(Cat#10667): 500mM Tris-HCl, 1M NaCl,100mM MgCl2, 1mg/mL OsrHSA, pH7.9@25℃
2）Incubate at 50°C 1 h;
3）DNA linearization is complete, and subsequent experiments can be performed.
Notes:
1. The volume of restriction endonuclease added should not exceed 1/10 of the reaction volume;
2. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB220514