HNRNPUL1抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
HNRNPUL1抗体

HNRNPUL1抗体

商家询价

产品名称: HNRNPUL1抗体

英文名称: HNRNPUL1Antibody

产品编号: XY10578-1-

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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 HNRNPUL1抗体

Applications
In-house applications ELISA, WB, IHC, IF, FC, IP     Application not tested? Please see our Guarantee.
Published applications IF, IP, WB Publications 5 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 10578-1-AP;A549 cellA549 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP(HNRNPUL1 antibody) at dilution of 1:2000
  • 10578-1-AP;Ovary tumorImmunohistochemical of paraffin-embedded human ovary tumor using 10578-1-AP(HNRNPUL1 antibody) at dilution of 1:100 (under 25x lens)
  • 10578-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using HNRNPUL1 antibody 10578-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 10578-1-AP;HeLa cellsIP Result of anti-HNRNPUL1 (IP:10578-1-AP, 5ug; Detection:10578-1-AP 1:2000) with HeLa cells lysate 2000ug.
  • 10578-1-AP;HepG2 cells1X10^6 HepG2 cells were stained with 0.2ug HNRNPUL1 antibody (10578-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in A549 cells, HEK-293 cells, HeLa cells, MCF7 cells, mouse brain tissue
Positive IP detected in HeLa cells
Positive IHC detected in Human ovary tumor tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:20-1:200
 HNRNPUL1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen HNRNPUL1 fusion protein ag0899 Full name Heterogeneous nuclear ribonucleoprotein U-like 1
Calculated molecular
weight
120 kDa Observed molecular 
weight
90-115 kDa
GenBank accession 
number
BC002564 Gene ID (NCBI) 11100
Gene symbol HNRNPUL1 Synonyms E1B AP5, E1BAP5, FLJ12944, HNRNPUL1, HNRPUL1
Background HNRNPUL1抗体

HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1–Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 . HNRNPUL1抗体

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