Recombinant Murine sRANK Ligand

Catalog Number:

315-11C

Synonyms:

soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254

Description:

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK + T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant Murine sRANK Ligand is a 19.8 kDa polypeptide comprising the TNF-homologous region of RANKL (178 amino acid residues).

AA Sequence:

FSGAPAMMEG SWLDVAQRGK PEAQPFAHLT INAASIPSGS HKVTLSSWYH DRGWAKISNM TLSNGKLRVN QDGFYYLYAN ICFRHHETSG SVPTDYLQLM VYVVKTSIKI PSSHNLMKGG STKNWSGNSE FHFYSINVGG FFKLRAGEEI SIQVSNPSLL DPDQDATYFG AFKVQDID

Source:

CHO cells

Purity:

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity:

Determined by its ability to activate LacZ reporter under control of NF-kappa-B-responsive element in RAW264.7 cells in the absence of any cross-linking. The expected ED₅₀ for this effect is 3.0-20.0 ng/ml.

Research Interests:

	Apoptosis		Bone, Skeletal, Cartilage
	Cancer		Diabetes / Weight Regulation
	Immune System		TNF Superfamily

Country of Origin:

USA