TurboFect 转染试剂
产品名称: TurboFect 转染试剂
英文名称: TurboFect Transfection Reagent
产品编号: R0533
产品价格: 0
产品产地: 美国
品牌商标: Thermo Fisher
更新时间: null
使用范围: null
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Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells. Features of TurboFect Transfection Reagent: High transfection efficiency of a wide variety of cell types. No optimization required for most cell lines. Excellent transfection efficiency in the presence or absence of serum. Minimal cytotoxicity. Ready-to-useno need to reconstitute, dilute or manipulate. Applications of TurboFect Transfection Reagent : Stable and transient transfection or co-transfection of DNA or RNA. Experiments with adherent or suspension cells, differentiated or undifferentiated cells. Gene expression studies and assays involving GFP or luciferase reporters. TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.