CENPB,着丝粒蛋白B抗体

产品编号HZ-6595R
英文名称CENPB
中文名称着丝粒蛋白B抗体
别 名CENP B; CENP-B; centromere autoantigen B; Centromere protein B; Major centromere autoantigen B; CENPB_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 染色质和核信号 细胞周期蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,
CENPB,着丝粒蛋白B抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量66kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CENPB (321-360aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CENPB,着丝粒蛋白B抗体PubMedPubMed
产品介绍background:
This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix loop helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17 bp sequence (CENPB box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. (referenced from Entrez Gene)

Function:
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Subunit:
Homodimer. Interacts with CENPT.

Subcellular Location:
Nucleus. Chromosome, centromere.

CENPB,着丝粒蛋白B抗体Similarity:
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain.

Database links:
UniProtKB/Swiss-Prot: P07199.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.