Anti-MAP Kinase 1/2 (Erk1/2) Rabbit Polyclonal Antibody-其它资料-资讯-生物在线

Anti-MAP Kinase 1/2 (Erk1/2) Rabbit Polyclonal Antibody

作者：上海康成生物工程有限公司 2008-01-30T00:00 (访问量:1597)

Anti-MAP Kinase 1/2 (Erk1/2) Rabbit Polyclonal Antibody

Catalog No: KC-5E01

Size: 100 µl (10 Western mini-blots)

Applications	Species Cross-Reactivity	Molecular Wt.	Source
W, IHC, F	H, M, R, Hm, Z, Ch, starfish	42, 44 kDa	Rabbit

Background:

Western blot analysis of extracts using p44/42 MAP Kinase Antibody.

Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neuro-transmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine 202 and 204 of Erk1 or 185 and 187 of rat Erk2 at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine. Upon activeation, the Erks either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activeate a number of transcription factors such as c-Fos and Elk-1.

Specificity/Sensitivity:

Erk 1/2 MAP Kinase Antibody detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not recognize either JNK/SAPK or p38 MAP kinase.

Source/Purification:

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to the C-terminal 35 amino acids of rat p44 MAP kinase. Antibodies are purified by protein A and peptide affinity chromatography.

Storage and Stability:

Each vial contains 100ul affinity purified rabbit anti-MAP Kinase 1/2 (Erk1/2) antibody in a special stabilizer. Real time studies that this special stabilizer helped retain most of antibody activity for 24 months while store at 4℃.

Recommended Antibody Dilutions:

Western blotting	1:1000
Immunohistochemistry (Paraffin)	1:100
Flow cytometry	1:25

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